Coding

Part:BBa_K2141002:Design

Designed by: Altug Uludag, Nedim Haciosmanoglu, Galip Can Guclu, Yagmur Guneri, Melis Akgun   Group: iGEM16_Istanbul_Tech   (2016-10-14)


ECFP-meth scFv-EYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 936
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We specially designed the construct with the antibody at the center that when methamphetamine binds to the antibody, ECFP and EYFP will create FRET effect.


Source

It is ordered from IDT sponsorship. ScFv comes from mus musculus anti-meth antibody, ECFP and EYFP are the engineered enhanced forms of YFP and CFP fluorescent proteins.

References